Journal: PloS one

Article Title: Association of interleukin-6 signalling with the muscle stem cell response following muscle-lengthening contractions in humans.

doi: 10.1371/journal.pone.0006027

Figure Lengend Snippet: Figure 1. Circulating IL-6 and muscle IL-6 mRNA and IL-6 receptor response muscle lengthening contractions (MLC). (1a) Average serum interleukin-6 (IL-6) concentration. Time 0 hr corresponds to pre-intervention values; all other time-points correspond to post-intervention time (hr). (1b) Relative IL-6 mRNA expression, expressed as fold-change from 0 hr. (1c) Pearson correlation of serum IL-6 concentration versus muscle IL-6 mRNA (fold-change), correlation is representative of the individual data points and presented as mean values ($)6SD (error bars). (1d) Relative IL-6 receptor (IL-6Ra) mRNA expression, expressed as fold-change from 0 hr. (1e) Immunofluorescent image (406) of a muscle cross-section triple-stained for Pax7 (red), IL-6Ra (green) and nuclei (DAPI = blue); IL-6Ra staining is apparent on the sarcolemma and satellite cell membrane. White arrow denotes one of the Pax7+ nuclei which co-localized with IL-6Ra (scale bar = 100 mm). Values are reported as mean6S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p,0.05 vs. 0 hr. doi:10.1371/journal.pone.0006027.g001

Article Snippet: Immunofluorescence 7 mm sections were cryosectioned and stained with antibodies against Pax7 (neat; DSHB, USA); IL-6 (500 ng/mL, MAB 2061, R&D Systems, USA); p-STAT3 (p-STAT3 Y705 1:100, Cell Signaling Technologies Inc., USA); IL-6Ra (1:50, MCA822, Serotec, UK); PCNA (1:200, ab15497, Abcam Inc., USA); and Laminin (1:1000, L8271, Sigma-Aldrich, Canada).

Techniques: Concentration Assay, Expressing, Staining, Membrane

Journal: International journal for parasitology

Article Title: Excretory/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and IL6 production

doi: 10.1016/j.ijpara.2015.06.001

Figure Lengend Snippet: IL6 production. IL6 production in normal human cholangiocytes (H69) and human colon cancer (Caco-2) co-cultured with Opisthorchis viverrini excretory-secretory products (OvES) in several dilutions for 48 h (A). The biliary cells were strongly stimulated by OvES protein, but had no effect in colon cancer cells when compared with control cells. IL6 production from normal human cholangiocyte cell (H69) with and without endocytosis inhibitors (chlorpromazine; CPZ, Filipin and Bafilomycin A1) before OvES stimulation for 48 h using the ELISA technique (B). OvES stimulated IL6 production while all inhibitors of endocytosis significantly suppressed the levels of IL6 secreted. Histograms represent the average of three independent experiments ± S.E.M. of the absorbance at 450 nm measured by a Versamax microplate reader using SoftMax pro V.5 program (*** P < 0.001).

Article Snippet: Supernatant was then collected and IL6 levels determined using a human IL6 ELISA kit (R&D Systems, USA) following the manufacturer’s recommendations.

Techniques: Cell Culture, Control, Enzyme-linked Immunosorbent Assay